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Updated: Aug 26, 2026
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
Automated detection of connective tissue by tissue counter analysis and classification and regression trees
1Department of Dermatology, University of Graz, Auenbruggerplatz 8, A-8036 Graz, Austria. josef.smolle@kfunigraz.ac.at
Objective:
To evaluate the feasibility of the CART (Classification and Regression Tree) procedure for the recognition of microscopic structures in tissue counter analysis.
Methods:
Digital microscopic images of H & E; stained slides of normal human skin and of primary malignant melanoma were overlayed with regularly distributed square measuring masks (elements) and grey value, texture and colour features within each mask were recorded. In the learning set, elements were interactively labeled as representing either connective tissue of the reticular dermis, other tissue components or background. Subsequently, CART models were based on these data sets.
Results:
Implementation of the CART classification rules into the image analysis program showed that in an independent test set 94.1% of elements classified as connective tissue of the reticular dermis were correctly labeled. Automated measurements of the total amount of tissue and of the amount of connective tissue within a slide showed high reproducibility (r=0.97 and r=0.94, respectively; p<0.001).
Conclusions:
CART procedure in tissue counter analysis yields simple and reproducible classification rules for tissue elements.
