Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p14(ARF) regulates E2F activity.

Sarah L Mason1, Oonagh Loughran, Nicholas B La Thangue

  • 1Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK.

Oncogene
|June 26, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Separate transcription and splicing gene networks are linked and coordinated by the pRb-E2F pathway.

Nucleic acids research·2026
Same author

rs6971 TSPO polymorphism in Parkinson's disease.

Movement disorders : official journal of the Movement Disorder Society·2025
Same author

Citrullination and the protein code: crosstalk between post-translational modifications in cancer.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2023
Same author

Long non-coding RNA-derived peptides are immunogenic and drive a potent anti-tumour response.

Nature communications·2023
Same author

The HDAC inhibitor zabadinostat is a systemic regulator of adaptive immunity.

Communications biology·2023
Same author

Case Report: A Novel Lateral Approach to the C7, C8, and T1 Intervertebral Foramina for Resection of Malignant Peripheral Nerve Sheath Neoplasia, Followed by Adjunctive Radiotherapy, in Three Dogs.

Frontiers in veterinary science·2022
Same journal

SRD5A3-mediated aberrant N-glycosylation of SCARA5 promotes ferroptosis in lung adenocarcinoma.

Oncogene·2026
Same journal

Aberrant splicing in human cancer shows possible functional impact on transcription factors.

Oncogene·2026
Same journal

The crosstalk between RNA m6A modification and protein lactylation: emerging insights into tumor progression.

Oncogene·2026
Same journal

Correction: Neuropilin-1 promotes human glioma progression through potentiating the activity of the HGF/SF autocrine pathway.

Oncogene·2026
Same journal

Amphiregulin-mediated EGFR activation drives both intrinsic and acquired resistance to KRAS G12C inhibitors in KRAS G12C-mutant non-small cell lung cancer.

Oncogene·2026
Same journal

Histone lactylation-driven IGF2BP3 promotes intrahepatic cholangiocarcinoma progression via SPP1/CD44-dependent macrophage polarization.

Oncogene·2026
See all related articles

The ARF protein (Alternative Reading Frame) regulates cell growth by influencing both the p53 and E2F pathways. This study shows ARF directly controls E2F transcription factor activity, impacting cell cycle arrest.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • The ARF protein, a product of the ink4a/arf locus, is induced by oncogenic signals.
  • ARF promotes cell growth arrest via the p53 pathway by inhibiting MDM2-mediated p53 downregulation.

Purpose of the Study:

  • To investigate if human p14(ARF) interacts with growth-regulating pathways beyond p53.
  • To determine if p14(ARF) directly influences E2F transcription factor activity.

Main Methods:

  • Cell-type specific analysis including p53(-/-)/mdm(-/-) MEFs.
  • Mutational analysis of p14(ARF) to distinguish binding domains.
  • Assessing E2F-dependent transcription and apoptosis.

Main Results:

Related Experiment Videos

  • p14(ARF) directly regulates E2F activity across different cell types, independent of MDM2.
  • p14(ARF) down-regulates E2F-dependent transcription and induces cell cycle arrest in cells undergoing E2F-dependent apoptosis.
  • Distinct binding domains for E2F-1 and MDM2 were identified on p14(ARF).

    • Conclusions:

    • p14(ARF) acts as a pleiotropic regulator of cell growth.
    • p14(ARF) targets both the E2F and p53 pathways, highlighting their interplay in proliferation control.