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Improved demonstration of mast cells using alcian blue tetrakis (methylpyridium) chloride
1Histopathology Department, The Children's Hospital at Westmead, Australia. anthonyh@chw.edu.au
Abstract:
Many batches of alcian blue dye are incompletely soluble at the low pH used for demonstrating mast cells. An improved technique using alcian blue tetrakis (methylpyridium) chloride (alcian blue pyridine variant) is described here. It produces stronger mast cell staining than other alcian blue stains tested.