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Related Experiment Videos

Tissue factor expression on monocyte subpopulations during normal pregnancy.

Valerie A Holmes1, Julie M W Wallace, W S Gilmore

  • 1Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine, UK. valerie.holmes@bll.n-i.nhs.uk

Thrombosis and Haemostasis
|June 27, 2002
PubMed
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Pregnancy reduces monocyte tissue factor (TF) expression, a key clotting initiator. This finding helps explain how the body maintains blood clotting balance during pregnancy.

Area of Science:

  • Hematology
  • Immunology
  • Reproductive Biology

Background:

  • Pregnancy is characterized by a hypercoagulable state, increasing thrombosis risk.
  • Tissue factor (TF) initiates the blood coagulation cascade.
  • Monocytes play a role in hemostasis and inflammation.

Purpose of the Study:

  • To investigate the impact of normal pregnancy on monocyte tissue factor (TF) expression.
  • To compare TF expression in monocytes of pregnant women versus non-pregnant controls.

Main Methods:

  • Longitudinal case-control study involving 89 pregnant and 39 non-pregnant women.
  • Whole blood flow cytometry used to measure TF expression.
  • CD14 identified monocytes, with TF expression analyzed on CD14 positive, CD14Bright, and CD14Dim subsets.

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Main Results:

  • Monocyte TF expression was significantly lower in pregnant women compared to controls at multiple gestational points (20 and 35 weeks).
  • Specific reductions were observed on CD14 positive cells (20, 35 weeks), CD14Bright cells (12, 35 weeks), and CD14Dim cells (20 weeks).
  • A higher proportion of CD14Dim monocytes expressed TF than CD14Bright monocytes.

Conclusions:

  • Monocyte TF expression is downregulated during normal pregnancy.
  • This reduction in TF expression may be a mechanism to maintain hemostatic balance in the hypercoagulable state of pregnancy.
  • Findings suggest a complex regulation of coagulation during gestation.