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Bacteria-modified amperometric immunosensor for a Brucella melitensis antibody assay
Zhi-Zhang Li1, Fu-Chun Gong, Guo-Li Shen
1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P R China.
Summary
A new immunosensor detects Brucella melitensis antibodies using horseradish peroxidase (HRP) and a graphite biocomposite. This sensitive biosensor offers a viable method for clinical diagnosis of Brucella infections.
Area of Science:
- Biosensors and Immunoassays
- Analytical Chemistry
- Microbiology
Background:
- Brucellosis remains a significant global health concern, necessitating sensitive and rapid diagnostic tools.
- Current diagnostic methods for Brucella infection can be time-consuming or require specialized laboratory equipment.
- Development of novel electrochemical immunosensors offers a promising avenue for point-of-care diagnostics.
Purpose of the Study:
- To develop and characterize a novel amperometric immunosensor for the detection of Brucella melitensis antibodies.
- To evaluate the performance of the immunosensor using a competitive binding assay in serum samples.
- To demonstrate the feasibility of the proposed biosensor for clinical Brucella diagnosis.
Main Methods:
- Fabrication of a biocomposite immunosensor using graphite powder, Brucella melitensis antigen, and silicate polymer gel.
- Immobilization of horseradish peroxidase (HRP)-labeled antibodies for signal amplification.
- Amperometric detection of the enzymatic product (o-aminophenol) at -150 mV vs. SCE.
Main Results:
- The developed immunosensor exhibited a low detection limit of 3.5 ng/ml for Brucella melitensis antibody.
- A linear detection range was established from 3.5 ng/ml to 200 ng/ml.
- The sensor successfully detected Brucella melitensis antibodies in serum samples using a competitive binding assay.
Conclusions:
- The novel amperometric immunosensor demonstrates high sensitivity and a broad linear range for detecting Brucella melitensis antibodies.
- The use of a graphite-based biocomposite and HRP labeling enhances sensor performance.
- The proposed immunosensor is a feasible and promising tool for the clinical diagnosis of Brucellosis.