Related Experiment Video
Updated: Sep 18, 2026

Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
Published on: June 24, 2016
[Regulation of lysine biosynthesis in the leucine-dependent mutant of Micrococcus glutamicus]
Abstract:
The role of leucine in metabolism of Micrococcus glutamicus was examined in relation to the lysis synthesis by the homoserine- and leucine-dependent strains of M. glutamicus 106 and the homoserine-dependent strain of M. glutamicus 95. In addition to the growth function, leucine produced a controlling effect on the yield of the end product. In the presence of leucine the inhibitory effect of isoleucine on the lysine yield was reduced or reversed. The end effect depended on the leucine: isoleucine ratio. The mechanism of interaction of amino acid metabolites with respect to the lysine biosynthesis in both strains is discussed.
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Amino Acid Biosynthetic Pathways
Biosynthesis of Nucleic Acids
Inducible Operons: lac Operon
Gene Regulation in Microbial Communities: Quorum Sensing
Stringent Response in E. coli

