Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High throughput genotyping technologies for pharmacogenomics.

M D Brennan1

  • 1Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, Kentucky 40292, USA. mdbren01@louisville.edu

American Journal of Pharmacogenomics : Genomics-Related Research in Drug Development and Clinical Practice
|June 27, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Professionalism and academic medicine: the Mayo Clinic program in professionalism.

Irish journal of medical science·2007
Same author

Thyroid hormones and illness.

Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·2004
Same author

The gypsy insulator can act as a promoter-specific transcriptional stimulator.

Molecular and cellular biology·2001
Same author

Polarity of transcriptional enhancement revealed by an insulator element.

Proceedings of the National Academy of Sciences of the United States of America·2000
Same author

Polymorphic markers for the arylsulfatase A gene reveal a greatly expanded meiotic map for the human 22q telomeric region.

Genomics·2000
Same author

Cis-acting sequences contributing to expression of the Drosophila affinidisjuncta Adh gene in both larvae and adults.

Insect biochemistry and molecular biology·1998
Same journal

Oncogenes as novel targets for cancer therapy (part IV): regulators of the cell cycle and apoptosis.

American journal of pharmacogenomics : genomics-related research in drug development and clinical practice·2005
Same journal

Using bioinformatics for drug target identification from the genome.

American journal of pharmacogenomics : genomics-related research in drug development and clinical practice·2005
Same journal

Antimycotic drug discovery in the age of genomics.

American journal of pharmacogenomics : genomics-related research in drug development and clinical practice·2005
Same journal

Cancer diagnostics: decision criteria for marker utilization in the clinic.

American journal of pharmacogenomics : genomics-related research in drug development and clinical practice·2005
Same journal

The Personalized Medicine Coalition: goals and strategies.

American journal of pharmacogenomics : genomics-related research in drug development and clinical practice·2005
Same journal

Expectations from structural genomics revisited: an analysis of structural genomics targets.

American journal of pharmacogenomics : genomics-related research in drug development and clinical practice·2005
See all related articles

Understanding genetic variability is key for personalized medicine. Advanced high-throughput methods rapidly score gene variants like single nucleotide polymorphisms (SNPs) for better disease and drug response insights.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Individual genetic differences influence disease susceptibility and drug efficacy.
  • Identifying specific gene variants (alleles) linked to clinical outcomes is a major challenge.
  • High-throughput, automated methods are crucial for analyzing genetic variability.

Purpose of the Study:

  • To review current and emerging technologies for rapid genetic analysis.
  • To highlight advancements in detecting microsatellite alleles and single nucleotide polymorphisms (SNPs).
  • To discuss methods improving efficiency, sensitivity, and cost-effectiveness in genotyping.

Main Methods:

  • Utilizes various detection technologies such as electrophoresis, fluorescence-based methods (polarization, FRET), and mass spectrometry.

Related Experiment Videos

  • Employs diverse SNP genotyping techniques including primer extension, allele-specific hybridization, and ligation assays.
  • Focuses on automated, high-throughput scoring of genetic variations.

    • Main Results:

    • Multiple technologies enable rapid scoring of microsatellite alleles and SNPs.
    • Newer methods offer reduced sample handling, enhanced sensitivity, greater flexibility, and lower costs.
    • Developments in combining amplification and detection show promise for streamlined genotyping.

    Conclusions:

    • Automated, high-throughput genotyping technologies are advancing rapidly.
    • Innovations are making genetic analysis more accessible, sensitive, and cost-effective.
    • These advancements are critical for understanding genetic variability and its clinical implications.