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[Comparative study of moderate and virulent phages of mesophilous lactic acid streptococci]
Prikladnaia Biokhimiia I Mikrobiologiia
|November 1, 1975
Abstract:
A method has been developed to identify phage containing mesophilous lactic acid streptococci. The method is based on the chloroform treatment of young cultures. The isolated moderate phages P4St and P6St are morphologically identical and serologically related to one another and to virulent phages P1Sv, P2Sv, P3Sv, P12Sv, P13Sv that can be isolated in industrial phagolysis.
Insights
A new method using chloroform treatment effectively identifies phages infecting lactic acid streptococci. This aids in understanding and controlling bacterial populations in industrial settings.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Mesophilic lactic acid streptococci are crucial in food fermentation.
- Bacteriophages (phages) can cause significant issues in industrial fermentation by lysing these bacteria.
- Effective identification of phages is essential for managing phage-related problems.
Purpose of the Study:
- To develop a reliable method for identifying phages that infect mesophilic lactic acid streptococci.
- To characterize isolated phages and their relationship to known virulent phages.
Main Methods:
- A novel method involving the chloroform treatment of young bacterial cultures was employed.
- Morphological and serological analyses were conducted on isolated phages.
Main Results:
- The chloroform treatment method successfully identified phages associated with mesophilic lactic acid streptococci.
- Two moderate phages, P4St and P6St, were isolated and found to be morphologically identical.
- These isolated phages (P4St, P6St) showed serological relatedness to each other and to virulent phages (P1Sv, P2Sv, P3Sv, P12Sv, P13Sv) found in industrial phagolysis.
Conclusions:
- The chloroform treatment is an effective technique for isolating and identifying phages targeting lactic acid streptococci.
- Understanding the relationship between moderate and virulent phages is important for managing industrial phage contamination and ensuring consistent fermentation processes.