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The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors

M C Marty1, F Alliot, J Rutin

  • 1Centre National de la Recherche Scientifique, 15 rue de l'Ecole de Médecine 75270 Paris CEDEX 06 France.

Proceedings of the National Academy of Sciences of the United States of America
|June 27, 2002
PubMed

Insights

Myelin basic protein (MBP) gene products, specifically hemopoietic MBP (HMBP), are found in both the nervous and entire hemopoietic systems. HMBP proteins are present in various immune cells, including T cells, B cells, and myeloid lineage cells.

Area of Science:

  • Immunology
  • Neuroscience
  • Molecular Biology

Background:

  • Myelin basic proteins (MBP) are key components of the myelin sheath in the central and peripheral nervous systems.
  • Previous research indicated MBP-related transcripts in the bone marrow and immune system.
  • The long MBP gene, also known as "Golli-MBP," contains upstream exons (region 0') encoding novel transcripts.

Purpose of the Study:

  • To investigate the presence and distribution of hemopoietic MBP (HMBP) proteins within the hemopoietic system.
  • To characterize HMBP expression in various immune cell populations.
  • To determine if HMBP is expressed in hematopoietic stem and progenitor cells.

Main Methods:

  • Utilized antisera against recombinant HMBP proteins for immunoreactivity assays.
  • Analyzed protein presence in brain, thymus, and spleen.
  • Investigated HMBP mRNA and protein expression in T cells, B cells, myeloid cells, megakaryocytes, erythroblasts, and CD34+ bone marrow cells.

Main Results:

  • HMBP proteins (26-28 kDa) were detected in brain, thymus, and spleen.
  • HMBP proteins and transcripts were found in >95% of thymic T cells, mature T cells, and a majority of B lymphocytes.
  • HMBP proteins were present in all myeloid lineage cells, megakaryocytes, erythroblasts, and CD34+ bone marrow cells, with expression upregulated in proliferative cultures.

Conclusions:

  • The MBP gene produces products, HMBP, that are expressed throughout the hemopoietic system, not just the nervous system.
  • HMBP is a significant protein marker in diverse immune cell populations, including lymphoid and myeloid lineages.
  • HMBP expression in CD34+ bone marrow cells suggests a role in early hematopoiesis and immune cell development.

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