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Published on: May 27, 2022
Microdeletions in the Y chromosome of patients with idiopathic azoospermia
Akiyuki Shimizu1, Tomohiko Ichikawa, Noriyuki Suzuki
1Department of Urology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Aim:
To evaluate the occurrence and prevalence of microdeletions in the gamma chromosome of patients with azoospermia.
Methods:
DNA from 29 men with idiopathic azoospermia was screened by polymerase chain reaction (PCR) analysis with a set of gamma chromosome specific sequence-tagged sites (STSs) to determine microdeletions in the gamma chromosome.
Results:
Deletions in the DAZ (deleted in azoospermia) loci sgamma254 and sgamma255 were found in three patients with idiopathic azoospermia, resulting in an estimated frequency of deletions of 10.7% in idiopathic azoospermia men.
Conclusion:
We conclude that PCR analysis is useful for the diagnosis of microdeletions in the Y chromosome, which is important when deciding the suitability of a patient for assisted reproductive technology such as testicular sperm extracion-intracytoplasmic sperm injection (TESE-ICSI).
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