Related Experiment Video
Updated: Jul 31, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Published on: January 18, 2014
L-glutamic acid production in a continuous stirred tank bioreactor using coimmobilized bio-catalyst using a
N Prabhu1, J Sarat Chandra Babu, S Sundaram
1Department of Chemical Engineering, Regional Engineering College, Tiruchirappalli-620 015, India.
Abstract:
The production of L-Glutamic acid has been studied using coimmobilized whole cells of pseudomonas reptilivora and micrococcus glutamicus in a two litre Tokyo Rikakikai fermentor using glucose as selected production medium. The process was carried out at an optimum temperature of 32 degree Celsius and a pH of 7.2. The progress of the reaction was recorded using Dr. Ingold fluorosensor. The effect of initial substrate concentration, speed of agitation, volume ofcalcium alginate beads and aeration rate on the yield of glutamic acid has been investigated. It has been found that the acid production increases exponentially with substrate concentration, and mass transfer co-efficient varied linearly with aeration rate. The kinetic parameters also had been estimated.

