Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A mark in the core: silence no more!

Patrick D Varga-Weisz1, Jacob Z Dalgaard

  • 1Marie Curie Research Institute, The Chart, Oxted RH8 0TL, Surrey, UK.

Molecular Cell
|June 28, 2002
PubMed
Summary

A novel methyltransferase, Dot1p, targets histone H3 lysine 79 specifically in nucleosomes. This modification may serve as a mark for open chromatin regions, expanding the known histone modification landscape.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does chromatin function as a metabolite reservoir?

Trends in biochemical sciences·2022
Same author

Genome organization and chromatin analysis identify transcriptional downregulation of insulin-like growth factor signaling as a hallmark of aging in developing B cells.

Genome biology·2018
Same author

What is the underlying cause of type II diabetes? - Are cells protecting themselves against the reactivity of glucose?

Medical hypotheses·2017
Same author

The Rim15-endosulfine-PP2ACdc55 signalling module regulates entry into gametogenesis and quiescence via distinct mechanisms in budding yeast.

PLoS genetics·2014
Same author

Chromatin remodeling: a collaborative effort.

Nature structural & molecular biology·2014
Same author

Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I.

PLoS genetics·2013

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Epigenetics

Background:

  • The repertoire of histone modifications is crucial for regulating gene expression.
  • Histone modifications play a key role in chromatin structure and function.

Purpose of the Study:

  • To introduce and characterize Dot1p, a novel methyltransferase.
  • To investigate the substrate specificity and context-dependency of Dot1p activity.
  • To explore the potential role of Dot1p-mediated methylation in chromatin organization.

Main Methods:

  • Biochemical assays to determine methyltransferase activity.
  • Analysis of histone modification patterns in vitro and in vivo.
  • Chromatin immunoprecipitation (ChIP) to assess chromatin accessibility.

Main Results:

  • Dot1p was identified as a novel methyltransferase.
  • Dot1p specifically methylates lysine 79 (K79) on histone H3.
  • This methylation occurs exclusively in the nucleosomal context.

Conclusions:

  • Dot1p expands the known histone modification repertoire.
  • Histone H3 lysine 79 methylation by Dot1p may function as a mark for open chromatin.
  • Dot1p activity is dependent on the nucleosomal context, suggesting a role in dynamic chromatin regulation.

Related Experiment Videos