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Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)

Son Radu1, Micky Vincent, Kasing Apun

  • 1Department of Biotechnology, Faculty of Food Science and Biotechnology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. son@fsb.upm.edu.my

Acta Tropica
|June 29, 2002
PubMed

Insights

Multidrug-resistant Vibrio cholerae O1 strains showed high but variable resistance during an outbreak. Molecular analysis revealed multiple clones were likely responsible for the cholera cases.

Area of Science:

  • Microbiology
  • Epidemiology
  • Molecular Biology

Background:

  • Antimicrobial resistance is a growing concern, particularly in areas with high antibiotic use.
  • Vibrio cholerae O1 is a significant cause of bacterial epidemics and pandemics.
  • Understanding the genetic diversity and resistance patterns of V. cholerae O1 is crucial for outbreak control.

Purpose of the Study:

  • To investigate the antimicrobial susceptibility patterns of Vibrio cholerae O1 during an outbreak.
  • To analyze the molecular diversity of V. cholerae O1 strains using Random Amplified Polymorphic DNA (RAPD) analysis.
  • To determine the clonal relationships and potential genetic factors contributing to virulence and resistance.

Main Methods:

  • Isolation and identification of Vibrio cholerae O1 from patient samples during an outbreak.
  • Antimicrobial susceptibility testing to assess resistance patterns.
  • Molecular typing using Random Amplified Polymorphic DNA (RAPD) analysis.
  • Detection of virulence genes (tcp, ctx, zot, ace).

Main Results:

  • High but variable rates of multidrug resistance were observed in V. cholerae O1 isolates.
  • Thirty-two of 33 isolates harbored key virulence genes (tcp, ctx, zot, ace).
  • RAPD analysis revealed genetic diversity, with isolates clustering into four main groups, suggesting multiple clones were involved.

Conclusions:

  • Multiple V. cholerae O1 clones appear to have contributed to the observed outbreak.
  • No direct correlation was found between specific RAPD patterns and antibiotic susceptibility profiles.
  • The presence of virulence genes suggests their role in the pathogenicity of outbreak strains.

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