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Related Experiment Videos

Monitoring and control of pullulan production using vision sensor.

M Ronen1, H Guterman, Y Shabtai

  • 1Unit of Biotechnology, Faculty of Engineering Sciences, Ben Gurion University of the Negev, P.O. Box 653, 84105, Beer-Sheva, Israel. michal.ronen@weizmann.ac.il

Journal of Biochemical and Biophysical Methods
|June 29, 2002
PubMed
Summary

This study developed a novel control strategy for Aureobasidium pullulans fermentation, significantly enhancing pullulan production. The system optimizes yeast-like cell concentration, boosting both process productivity and yield.

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Area of Science:

  • Biotechnology
  • Microbial Fermentation
  • Bioprocess Engineering

Background:

  • Pullulan production by Aureobasidium pullulans involves cellular morphogenetic changes.
  • High pullulan productivity and yield correlate with high yeast-like cell concentration.
  • Cellular morphology is influenced by culture conditions like dissolved oxygen and shear rate.

Purpose of the Study:

  • To develop a novel control strategy for improving pullulan production.
  • To maintain high yeast-like cell concentration through a feeding strategy.
  • To enhance overall process productivity and yield.

Main Methods:

  • Formulation of a novel control law based on cellular composition.
  • Implementation of a feeding strategy triggered by yeast-like cell concentration.

Related Experiment Videos

  • Monitoring of cellular morphogenetic composition using a vision sensor.

    • Main Results:

    • The proposed control strategy significantly improved pullulan production.
    • Process productivity increased by 67%.
    • Cell yield increased by 80%.

    Conclusions:

    • The developed monitoring and control system effectively increases pullulan productivity and yield.
    • This approach demonstrates potential for optimizing other cellular bioprocesses.
    • Maintaining optimal yeast-like cell concentration is crucial for high-yield fermentation.