Related Experiment Videos
Simple quantification of Cu,Zn-superoxide dismutase activity after separation by non-denaturing isoelectric focusing
Youji Shimazaki1, Hiromi Ohnishi, Satoshi Matsuura
1Department of Material Science, Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama City, 790-8577 Japan. yoji@dpc.ehime-u.ac.jp
Biochimica Et Biophysica Acta
|July 2, 2002
Abstract:
The Cu,Zn-superoxide dismutase (SOD) activity in bovine retina cytosol was separated from retinal pigment using short-length non-denaturing isoelectric focusing (IEF) (15-mm long x 1.3-mm i.d. column) and detected using non-denaturing two-dimensional electrophoresis (2-DE). After the SOD and pigment in the retina cytosol are separated, SOD activity can be quantified by water-soluble tetrazolium salt. We also found that SOD separated by this IEF retained its native function.