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Modulation by nutrients and drugs of liver acyl-CoAs analyzed by mass spectrometry

B Kalderon1, V Sheena, S Shachrur

  • 1Department of Human Nutrition and Metabolism, Hebrew University Medical School, Jerusalem, Israel 91120.

Journal of Lipid Research
|July 2, 2002
PubMed

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NMR and Mass Spectroscopy of Carboxylic Acids01:30

NMR and Mass Spectroscopy of Carboxylic Acids

In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the disappearance of the acidic...