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Cloning and sequencing of the serine dehydrogenase gene from Agrobacterium tumefaciens
Hisae Fujisawa1, Shinji Nagata, Emran Kabir Chowdhury
1Department of Bioresources Science, Kochi University, Japan.
Bioscience, Biotechnology, and Biochemistry
|July 3, 2002
Abstract:
The structural gene for NADP+-dependent serine dehydrogenase [EC 1.1.1.-] from Agrobacterium tumefaciens ICR 1600 was cloned into Escherichia coli cells and its complete DNA sequence was analyzed. The gene encodes a polypeptide containing 249 amino acid residues. The enzyme had high sequence similarity to short-chain alcohol dehydrogenases from bacteria and unknown proteins of Haemophilus influenzae, Escherichia coli, and Saccharomyces cerevisiae.

