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Serum leptin concentrations after surgery in young rats
Helena Zivna1, Pavel Zivny, Vladimir Palicka
1Institute of Physiology, Charles University Prague, Faculty of Medicine and Faculty Hospital, CZ 5005 Hradec Kralove, Czech Republic. zivny@lfhk.cuni.cz
Nutrition (Burbank, Los Angeles County, Calif.)
|July 3, 2002
Summary
Surgical procedures in rats significantly decrease serum leptin levels. Ibuprofen treatment further reduces leptin, suggesting organs like the liver may clear leptin.
Area of Science:
- Endocrinology
- Surgical Physiology
- Pharmacology
Background:
- Leptin, a hormone regulating appetite and metabolism, can be influenced by physiological stressors.
- Surgical interventions and associated inflammation/pain may impact serum leptin concentrations.
Purpose of the Study:
- To investigate changes in serum leptin levels following various surgical procedures in young male rats.
- To evaluate the effect of ibuprofen, an anti-inflammatory drug, on serum leptin concentrations after surgery.
Main Methods:
- Serum leptin was measured using radioimmunoassay in rats before and 18 hours after laparotomy, partial hepatectomy, splenectomy, or unilateral nephrectomy.
- Some rats underwent ibuprofen treatment post-surgery.
- Blood samples were collected from multiple major vessels.
Main Results:
- Serum leptin significantly decreased after laparotomy and partial hepatectomy with ibuprofen treatment.
- Laparotomy alone also resulted in a significant decrease in serum leptin.
- No significant changes in leptin were observed after splenectomy or unilateral nephrectomy alone.
Conclusions:
- Surgical stress in rats is associated with reduced serum leptin concentrations.
- The liver, spleen, and kidney may play a role in leptin clearance.
- Ibuprofen administration exacerbates the decrease in serum leptin following surgery.