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Updated: Aug 17, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Determination and degradation study of haloperidol by high performance liquid chromatography
H Trabelsi1, S Bouabdallah, K Bouzouita
1Laboratoire National de Contrôle des Médicaments, 11 bis, rue jebel Lakhdar, Bab Saadoun 1006, Tunis, Tunisia. trhassen@rns.tn
Abstract:
A specific, high performance liquid chromatographic method was developed for the assay of haloperidol, together with an adequate separation of its degradation products. The method is based on the use of an octadecylsilane stationary phase column under isocratic conditions. The mobile phase consisted of 50 mM sodium phosphate monobasic pH 2.5-acetonitrile-THF-TEA (63:34:3:0.1, v/v/v/v) adjusted with o-phosphoric acid to a pH of 2.5. The degradation was performed in hydrochloric acid, sodium hydroxide and hydrogen peroxide. The main degradation products were identified. Application of the assay of haloperidol in tablet formulations is presented.
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