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Drosophila cyclin E interacts with components of the Brahma complex
Anthony M Brumby1, Claudia B Zraly, Julie A Horsfield
1Peter MacCallum Cancer Institute, Locked bag 1, A'Beckett Street, Melbourne, Victoria 8006, Australia.
The EMBO Journal
|July 3, 2002
Summary
The Brahma (Brm) complex suppresses entry into S phase by acting downstream of Cyclin E accumulation. This chromatin remodeling complex interacts with Cyclin E, impacting cell cycle progression.
Area of Science:
- Cell cycle regulation
- Chromatin remodeling
- Molecular genetics
Background:
- Cyclin E-Cdk2 is crucial for initiating S phase.
- Understanding genes that interact with Cyclin E is key to deciphering cell cycle control.
- The Brahma (Brm) complex is a conserved chromatin remodeler related to SWI-SNF.
Purpose of the Study:
- To identify genes interacting with Drosophila Cyclin E (DmcycE).
- To investigate the role of the Brm complex in cell cycle regulation.
- To elucidate the relationship between DmcycE and the Brm complex in S phase entry.
Main Methods:
- Genetic screen using a hypomorphic mutation of Drosophila cyclin E (DmcycE(JP)) to identify dominant suppressors.
- Analysis of Brm complex components (brm, mor, snr1, osa) and their effect on the DmcycE(JP) eye phenotype.
- In vivo co-immunoprecipitation to assess physical interactions between DmcycE, Brm, Snr1, and Rbf1.
Main Results:
- Mutations in Brm complex genes (brm, mor, snr1, osa) suppress the DmcycE(JP) rough eye phenotype.
- Brm complex mutants increase S phase entry without altering DmcycE protein levels.
- DmcycE physically interacts with Brm and Snr1 in vivo; Brm complex interacts weakly with Rbf1.
Conclusions:
- The Brm complex inhibits S phase entry, acting downstream of DmcycE accumulation.
- The Brm complex plays a role in regulating cell cycle progression, independent of Rbf1's role in G1 arrest.
- This study reveals a novel interaction between chromatin remodeling and cell cycle control mediated by Cyclin E.