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High-throughput Detection Method for Influenza Virus
10:05

High-throughput Detection Method for Influenza Virus

Published on: February 4, 2012

Bedside diagnosis of influenzavirus infections in hospitalized children

Katherine A Poehling1, Marie R Griffin, Robert S Dittus

  • 1Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA. katherine.poehling@mcmail.vanderbilt.edu

Pediatrics
|July 3, 2002
PubMed

Insights

The bedside QuickVue Influenza Test accurately identifies influenza in hospitalized children. Negative results strongly suggest no infection, while positive results increase the likelihood of influenza illness.

Area of Science:

  • Pediatric Infectious Diseases
  • Clinical Diagnostics
  • Virology

Background:

  • Nosocomial influenza infections pose a significant risk in healthcare settings.
  • Prompt diagnosis of influenza is crucial for effective antiviral therapy and infection control.
  • There is a need for accessible, rapid diagnostic methods for influenzavirus.

Purpose of the Study:

  • To evaluate the performance of the QuickVue Influenza Test, a lateral-flow immunoassay, for diagnosing influenza in hospitalized children.
  • To assess the utility of this rapid diagnostic test at the bedside during the influenza season.

Main Methods:

  • A prospective study was conducted on hospitalized children with respiratory symptoms or fever during the 1999-2000 influenza season.
  • Nasal swabs were collected for comparison between the QuickVue Influenza Test and traditional methods (culture and PCR).
  • The diagnostic accuracy of the QuickVue Influenza Test was analyzed against confirmed influenza A or B results.

Main Results:

  • The study enrolled 233 children, with a confirmed influenza A prevalence of 8%.
  • The QuickVue Influenza Test demonstrated high specificity (98%) and negative predictive value (98%).
  • Sensitivity and positive predictive value were 74% for the test.

Conclusions:

  • Negative QuickVue Influenza Tests in hospitalized children with fever/respiratory symptoms indicate a very low probability of influenza infection.
  • Positive QuickVue Influenza Tests significantly increase the probability of influenza-associated illness in this pediatric population.
  • The bedside lateral-flow immunoassay is a valuable tool for ruling out influenza in hospitalized children.
Abstract

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