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Related Experiment Videos

Biased Iglambda expression in hypermutated IgD multiple myelomas does not result from receptor revision.

M van der Burg1, R J Bende, W M Aarts

  • 1Dept of Immunology, Erasmus University Rotterdam/University Hospital Rotterdam, The Netherlands.

Leukemia
|July 3, 2002
PubMed
Summary

IgD multiple myelomas (MM) originate from germinal center B cells. Despite hypermutation, these cells show no evidence of light chain receptor revision, challenging previous theories.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • IgM(-)IgD(+) B cells and multiple myelomas (MM) exhibit Cmu deletion, Iglambda preference, and hypermutated IgV regions.
  • Predominant Iglambda usage is hypothesized to stem from secondary gene rearrangements during germinal center B cell expansion.

Purpose of the Study:

  • To investigate light chain receptor revision in IgDlambda MM using a single-cell model.
  • To analyze IGK and IGL alleles in four IgDlambda MM cases.

Main Methods:

  • Southern blotting
  • Reverse transcription polymerase chain reaction (RT-PCR)
  • DNA sequencing

Main Results:

  • Confirmed Cmu deletion in two cases and extensive IgV gene mutation.

Related Experiment Videos

  • Identified six unmutated, non-functional IGK rearrangements in the four MM cases.
  • Found no evidence of receptor revision in hypermutated IgDlambda MM cells.

    • Conclusions:

    • IgD myelomas originate from post-germinal center B cells.
    • Hypermutation occurs independently of receptor revision in these cells.
    • The study refutes the hypothesis of secondary Ig gene rearrangements for Iglambda selection in IgD MM.