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Related Experiment Videos

Resveratrol modulates rat macrophage functions.

J Leiro1, E Alvarez, D García

  • 1Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain. mpleiro@usc.es

International Immunopharmacology
|July 4, 2002
PubMed
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Trans-resveratrol (RESV), a grape polyphenol, significantly inhibits rat macrophage responses like reactive oxygen and nitrogen intermediate production. This suggests RESV may treat diseases linked to overactive macrophages.

Area of Science:

  • Immunology
  • Pharmacology
  • Natural Products Chemistry

Background:

  • Macrophages are key immune cells involved in pathogen defense.
  • Reactive oxygen intermediates (ROIs) and reactive nitrogen intermediates (RNIs) are crucial for macrophage antipathogen activity.
  • Trans-resveratrol (RESV), a polyphenol from grapes, possesses antioxidant properties.

Purpose of the Study:

  • To investigate the effects of RESV on rat macrophage respiratory-burst and phagocytic activities.
  • To determine the dose-dependent inhibitory effects of RESV on macrophage responses.

Main Methods:

  • Primary rat peritoneal macrophages were isolated.
  • Cells were stimulated with phorbol 12-myristate 13-acetate (PMA) or thioglycollate.
  • Respiratory-burst responses (ROI production) and phagocytic activity (uptake of Kluyveromyces lactis) were measured.

Related Experiment Videos

  • Production of RNIs was also assessed.

    • Main Results:

    • RESV (1-10 microM) dose-dependently inhibited extracellular and intracellular ROI production stimulated by PMA.
    • RESV (10-100 microM) inhibited RNI production stimulated by thioglycollate.
    • RESV (>10 microM) dose-dependently inhibited the phagocytosis of K. lactis by macrophages.

    Conclusions:

    • RESV is a potent inhibitor of rat macrophage antipathogen responses, including ROI and RNI production.
    • RESV also impairs macrophage phagocytic capacity at higher concentrations.
    • These findings suggest potential therapeutic applications for RESV in diseases characterized by macrophage hyperresponsiveness.