Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence and structure classification of kinases.

Sara Cheek1, Hong Zhang, Nick V Grishin

  • 1Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA.

Journal of Molecular Biology
|July 4, 2002
PubMed
Summary

This study classifies over 17,000 kinase sequences into 30 families, revealing that 19 families share seven common structural folds. This reveals links between kinase structure and function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Control of spiral breakup by an alternating advective field.

The Journal of chemical physics·2006
Same author

Inhibition of growth and metastasis of human hepatocellular carcinoma by antisense oligonucleotide targeting signal transducer and activator of transcription 3.

Clinical cancer research : an official journal of the American Association for Cancer Research·2006
Same author

Identification of key genes responsible for cytokine-induced erythroid and myeloid differentiation and switching of hematopoietic stem cells by RAGE.

Cell research·2006
Same author

New rod-plate anterior instrumentation for thoracolumbar/lumbar scoliosis: biomechanical evaluation compared with dual-rod and single-rod with structural interbody support.

Spine·2006
Same author

Dimeric ansamycins--a new class of antitumor Hsp90 modulators with prolonged inhibitory activity.

International journal of cancer·2006
Same author

A unified mode of epigenetic gene silencing: RNA meets polycomb group proteins.

RNA biology·2006

Area of Science:

  • Biochemistry
  • Structural Biology
  • Enzymology

Background:

  • Kinases are crucial enzymes catalyzing phosphoryl transfer, primarily from ATP.
  • Despite a conserved core reaction, kinases exhibit vast diversity in substrate specificity, structure, and biological pathways.
  • Understanding the link between structural fold and functional specificity is key to kinase research.

Purpose of the Study:

  • To comprehensively survey and classify all available kinase sequences.
  • To investigate the relationship between structural folds and functional specificities in kinases.
  • To analyze shared and varied substrate binding and catalytic mechanisms within kinase fold groups.

Main Methods:

  • Performed a comprehensive sequence similarity survey of over 17,000 kinase sequences.

Related Experiment Videos

  • Classified kinases into 30 distinct families based on sequence similarities.
  • Mapped kinase families to known three-dimensional structural folds.

    • Main Results:

    • Identified 30 distinct kinase families, with 19 families (98% of sequences) belonging to seven general structural folds.
    • These folds include widespread types like Rossmann, ferredoxin, ribonuclease H, and TIM beta/alpha-barrel.
    • Examined conserved and divergent substrate binding and catalytic mechanisms across different fold groups.

    Conclusions:

    • Kinase classification based on sequence similarity reveals a strong correlation with structural folds.
    • Structural folds provide a framework for understanding conserved and varied kinase functions.
    • Identified instances of convergent evolution where identical kinase activities evolved in different structural folds.