Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Restriction landmark genome scanning.

Joseph F Costello1, Dominic J Smiraglia, Christoph Plass

  • 1University of California-San Francisco, San Francisco, CA 94115-0875, USA. jfcostello@cc.ucsf.edu

Methods (San Diego, Calif.)
|July 4, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nuclear-specific reductive carboxylation of alpha-ketoglutarate fuels histone acetylation to induce chromatin accessibility and gene activation.

Nature communications·2026
Same author

Integrated multi-omic profiling reveals two distinct splenic marginal zone lymphoma subgroups with prognostic relevance.

Blood advances·2026
Same author

Mutant IDH1 blocks neutropoiesis by repressing myeloid progenitor programs.

Blood·2026
Same author

GLYATL1 is associated with metabolic and epigenetic changes and with endocrine resistance in luminal breast cancer.

Clinical epigenetics·2026
Same author

ID3 deficiency alters chromatin accessibility at DSB sites and enhances vulnerability to HDAC inhibition.

International journal of cancer·2026
Same author

Epigenetic dysregulation of IRF9 drives excessive interferon signaling in COPD.

EMBO molecular medicine·2026
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Restriction landmark genome scanning (RLGS) is a quantitative method to analyze DNA methylation across thousands of CpG islands. This technique aids in identifying gene alterations in cancer and understanding their genomic influence.

Area of Science:

  • Genomics
  • Epigenetics
  • Cancer Research

Background:

  • CpG islands are crucial regulatory regions in the genome.
  • DNA methylation patterns are altered in various cancers.
  • Simultaneous analysis of thousands of CpG sites is needed for comprehensive genomic studies.

Purpose of the Study:

  • To introduce and detail Restriction landmark genome scanning (RLGS) as a quantitative method.
  • To highlight RLGS's capability in simultaneously assessing methylation status of numerous CpG islands.
  • To showcase RLGS's utility in cancer research and genomic analysis.

Main Methods:

  • RLGS employs two-dimensional separation of radiolabeled restriction enzyme fragments (e.g., NotI).
  • It distinguishes single-copy CpG islands from multicopy sequences.

Related Experiment Videos

  • Utilizes the methylation-sensitive endonuclease activity of NotI for differential methylation analysis.

    • Main Results:

    • RLGS successfully identifies novel imprinted genes.
    • It detects DNA amplification and methylation targets in human cancer.
    • The method identified deletion, methylation, and gene amplification in a mouse cancer model.

    Conclusions:

    • RLGS is a powerful tool for high-throughput methylation analysis.
    • It enables pattern recognition across tumor types and estimates methylation's influence on cancer genomes.
    • RLGS integrates methylation analysis with gene copy number analysis.