Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-specific DNA cleavage on surfaces.

Manchun Lu1, Jeff G Hall, Michael R Shortreed

  • 1Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706-1396, USA.

Journal of the American Chemical Society
|July 4, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid Peptide Mapping of Monoclonal Antibodies with Direct Infusion Mass Spectrometry.

bioRxiv : the preprint server for biology·2026
Same author

Directed evolution of APOX for proximity labeling using phenols with high redox potentials.

Cell chemical biology·2026
Same author

Interactome screening implicates BAG6 as a suppressor of UBQLN2 misfolding in ALS/FTD.

Frontiers in molecular neuroscience·2026
Same author

"The sky on our shoulders": a qualitative study of family caregivers' psychosocial experiences and unmet palliative care needs in advanced lung cancer.

BMC palliative care·2026
Same author

DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separation.

The Journal of biological chemistry·2026
Same author

Interactome screening implicates BAG6 as a suppressor of UBQLN2 misfolding in ALS-dementia.

bioRxiv : the preprint server for biology·2025
Same journal

Proton-Gated Torsional Spring for Molecular Energy Storage.

Journal of the American Chemical Society·2026
Same journal

Topologically Programmed Dual-Channel Covalent Organic Frameworks Decouple Gas and Ion Fluxes for Acidic CO<sub>2</sub> Electroreduction.

Journal of the American Chemical Society·2026
Same journal

Plasmonic Re-Excitation Enables Superoxide-Mediated Ethane Conversion to Acetic Acid under Visible Light.

Journal of the American Chemical Society·2026
Same journal

Photocatalytic Controlled Halodefluorination of Perfluoroalkyl Compounds Using <i>N</i>-Arylphenothiazines.

Journal of the American Chemical Society·2026
Same journal

Photoinduced Disproportionation Enables Oxidative Addition of Aryl Iodides at a Gallium(I) Center.

Journal of the American Chemical Society·2026
Same journal

Biocatalytic C3 β-<i>O</i>-Glycosylation of Triterpenes and Sterols to Synthesize Natural and Unnatural Saponins.

Journal of the American Chemical Society·2026
See all related articles

This study introduces a novel method combining invasive cleavage assays with DNA arrays for efficient single nucleotide polymorphism (SNP) detection. This approach enables sensitive and specific SNP scoring directly from genomic DNA without amplification.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Single nucleotide polymorphisms (SNPs) are crucial genetic markers.
  • Existing SNP detection methods often require target amplification.
  • A need exists for sensitive, specific, and scalable SNP detection techniques.

Purpose of the Study:

  • To develop a novel approach for large-scale SNP scoring using invasive cleavage assays and surface DNA arrays.
  • To evaluate two surface-based invasive cleavage reaction strategies.
  • To demonstrate unambiguous discrimination of single base differences.

Main Methods:

  • Integration of structure-specific invasive cleavage reactions with surface DNA array technology.
  • Design and implementation of two surface invasive cleavage reaction strategies.

Related Experiment Videos

  • Testing on a model SNP system in the human ApoE gene using PCR-amplified genomic DNA.

    • Main Results:

    • Demonstrated unambiguous discrimination of single base differences in SNPs.
    • Successfully implemented surface-based invasive cleavage assays.
    • Developed a theoretical model that accurately predicted experimental results.

    Conclusions:

    • The integrated invasive cleavage assay and surface DNA array technology offers a sensitive and specific method for SNP detection.
    • This parallel format approach is suitable for potentially large-scale SNP scoring.
    • The method allows direct detection from genomic DNA, eliminating the need for prior amplification.