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Transcriptional regulation of nuclear orphan receptor, NOR-1, by Ca(2+)/calmodulin-dependent protein kinase cascade
Hiroyuki Inuzuka1, Hiroshi Tokumitsu, Naganari Ohkura
1Department of Chemistry, Kagawa Medical University, 1750-1 Miki-cho, Kita-gun, Japan. tokumit@kms.ac.jp
Abstract:
We found that transcription of NOR-1 (neuron-derived orphan receptor-1) is induced by the Ca(2+)/calmodulin-dependent protein kinase IV (CaM-KIV) cascade by differential hybridization screening of a cDNA array using probes prepared from SH-SY5Y cells infected with recombinant adenoviruses expressing Ca(2+)/CaM-independent mutants of CaM-KIV and CaM-K kinase (CaM-KK). Induction of NOR-1 mRNA expression by the CaM-KIV cascade was confirmed by RT-PCR. Promoter analyses indicate that CaM-KIV cascade response element is located between -162 bp and -42 bp in the 1.7 kb NOR-1 promoter containing triple cAMP response elements. Disruption of each element significantly reduced the promoter activity, indicating the direct transcriptional regulation of NOR-1 by CaM-KIV cascade.
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