Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Near-membrane iminocoumarin-based low affinity fluorescent Ca(2+) indicators.

F Liepouri1, T G Deligeorgiev, Z Veneti

  • 1Department of Chemistry, University of Crete, Heraklion, 71 409 Crete, Greece.

Cell Calcium
|July 6, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enantioselective synthesis of a costic acid analogue with acaricidal activity against the bee parasite <i>Varroa destructor</i>.

Royal Society open science·2020
Same author

Reporting effectiveness of an extract of three traditional Cretan herbs on upper respiratory tract infection: results from a double-blind randomized controlled trial.

Journal of ethnopharmacology·2015
Same author

Automated recovery of the center of rotation in optical projection tomography in the presence of scattering.

IEEE journal of biomedical and health informatics·2012
Same author

Loss of reproductive parasitism following transfer of male-killing Wolbachia to Drosophila melanogaster and Drosophila simulans.

Heredity·2012
Same author

The transformer gene in Bactrocera oleae: the genetic switch that determines its sex fate.

Insect molecular biology·2007
Same author

Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker.

Insect molecular biology·2006
Same journal

Calorie restriction modulates beta cell IP<sub>3</sub>R activity to regulate Ca<sup>2+</sup> homeostasis and cell network connectivity.

Cell calcium·2026
Same journal

Spatially multiplexed stimulation reveals nonlinear calcium signaling in platelets.

Cell calcium·2026
Same journal

Calcium handling properties and arrhythmia vulnerability of cardiomyocytes from dystrophin-deficient mdx mice.

Cell calcium·2026
Same journal

Molecular actors of the Calcium signaling in the endothelial-to-mesenchymal transition: poorly explored therapeutic targets.

Cell calcium·2026
Same journal

High-throughput quantitation of pathogen-induced calcium signals captured through live-cell fluorescence microscopy.

Cell calcium·2026
Same journal

A model for the complete sequence of the human voltage-gated calcium channel (Ca<sub>V</sub>1.2) in a lipid bilayer.

Cell calcium·2026
See all related articles

Two novel fluorescent calcium (Ca2+) indicators were developed for near-membrane applications. These probes exhibit the highest Ca2+ dissociation constants reported, enabling sensitive detection of low calcium levels.

Area of Science:

  • Chemical Biology
  • Molecular Imaging
  • Biochemistry

Background:

  • Accurate measurement of intracellular calcium (Ca2+) is crucial for understanding cellular signaling.
  • Developing sensitive and specific fluorescent probes is essential for monitoring Ca2+ dynamics.
  • Existing probes may have limitations in affinity or localization for specific biological contexts.

Purpose of the Study:

  • To synthesize and characterize novel iminocoumarin-based fluorescent probes for near-membrane calcium (Ca2+) detection.
  • To evaluate the spectral properties and calcium binding characteristics of the new indicators.
  • To assess the potential of these probes as ratiometric indicators for low-affinity Ca2+ sensing.

Main Methods:

  • Synthesis of two iminocoumarin-based fluorescent compounds incorporating BAPTA structures.

Related Experiment Videos

  • Spectroscopic analysis of the free and Ca2+-bound forms of the synthesized probes.
  • Determination of Ca2+ dissociation constants (Kd) and evaluation of spectral shifts upon Ca2+ binding.

    • Main Results:

    • Successful synthesis of two novel near-membrane fluorescent Ca2+ indicators.
    • Probes exhibit high Ca2+ dissociation constant values (5.50 and 4.49 µM), the highest reported to date.
    • Fluorescence spectra show distinct shifts in excitation maxima and intensity changes upon Ca2+ binding, suitable for ratiometric measurements.

    Conclusions:

    • The newly synthesized iminocoumarin-based probes are effective fluorescent indicators for near-membrane Ca2+.
    • Their high Ca2+ dissociation constants make them suitable for detecting low Ca2+ concentrations.
    • These probes offer potential for ratiometric imaging of Ca2+ dynamics in specific cellular compartments.