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Updated: Aug 14, 2026

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
Published on: February 13, 2019
Fluorescent 1,10-phenanthroline-containing oligonucleotides distinguish between perfect and mismatched base pairing
Dennis J Hurley1, Susan E Seaman, Jan C Mazura
1Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA.
Abstract:
[structure: see text] A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dU(phen) duplexes are characteristic of the base opposite dU(phen) and distinguish between perfect and mismatched complementary oligonucleotides.
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