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Expression of beta-1,4-galactosyltransferase II and V in rat injured sciatic nerves
Aiguo Shen1, Hanzhou Wang, Yong Zhang
1Box 103, Gene Research Center, Medical Center of Fudan University (Former Shanghai Medical University), 200032, Shanghai, People's Republic of China.
Abstract:
Beta-1,4-Galactosyltransferases II and V (beta-1,4-GalT II and V) are involved in the biosynthesis of N-linked oligosaccharides as beta-1,4-GalT I which plays important roles in promoting neuron outgrowth. In the present paper, it was illustrated that beta-1,4-GalT II and V were localized mainly in Schwann cells of lesion sciatic nerves by in situ hybridization. Northern blot showed that the expression of beta-1,4-GalT II increased gradually at both stumps of injured nerves, while that of beta-1,4-GalT V decreased at proximal stumps but increased and reached its peak on the third day post-operation at distal stumps, before it declined. The different expression of beta-1,4-GalT II and V in Schwann cells suggested that they would affect the different galactosylation of glycoproteins in injured nerves regeneration.