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Updated: Aug 15, 2026

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
Published on: February 12, 2014
Nonperturbing fluorescent labeling of polysaccharides
Françoise Meunier1, Kevin J Wilkinson
1CABE (Analytical and Biophysical Environmental Chemistry), Sciences II, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
Abstract:
A fluorescent labeling procedure, which does not perturb macromolecular conformations, was employed to bind a rhodamine derivative to the reducing end of several water-soluble polysaccharides by reductive amination in the presence of sodium cyanoborohydride. Fluorescence correlation spectroscopy, atomic force microscopy, and size exclusion chromatography were used to demonstrate that the conformations of the polysaccharides schizophyllan, polygalacturonic acid (PGUA), succinoglycan, and several dextrans were maintained following the labeling procedure.
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