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Expansion of CD5 - B cells in multiple sclerosis correlates with CD80 (B7-1) expression
F Sellebjerg1, J Jensen, C V Jensen
1Department of Neurology, University of Copenhagen, Glostrup Hospital, Denmark. sellebjerg@dadlnet.dk
Scandinavian Journal of Immunology
|July 9, 2002
Summary
In multiple sclerosis (MS), CD5- B cells are expanded in the cerebrospinal fluid (CSF). This contrasts with CD5+ B cells, which may correlate with lower anti-myelin antibody production in MS patients.
Area of Science:
- Neuroimmunology
- B-cell biology
- Autoimmunity
Background:
- The role of autoantibodies in multiple sclerosis (MS) pathogenesis remains unclear.
- CD5 expression on B cells is linked to autoantibody production and B-cell tolerance mechanisms.
Purpose of the Study:
- To investigate the association between B-cell subsets, autoantibody production, and disease characteristics in MS.
- To explore the role of CD5+ and CD5- B cells in the cerebrospinal fluid (CSF) of MS patients.
Main Methods:
- Analysis of B-cell subsets (CD5+, CD5-) in CSF from patients with clinically isolated syndromes (CIS) and clinically definite MS (CDMS) compared to controls.
- Quantification of anti-myelin basic protein (anti-MBP) antibody-secreting cells in CSF.
- Assessment of serum autoantibodies and CSF immunoglobulin G (IgG) oligoclonal bands.
Main Results:
- Patients with CDMS showed a higher percentage of CD5- B cells in CSF compared to controls.
- CIS patients with specific MRI or CSF findings had increased CD5- B cells in CSF.
- Higher percentages of CD5- B cells correlated with increased CSF anti-MBP antibody-secreting cells, while CD5+ B cells showed a negative correlation.
Conclusions:
- Intrathecal expansion of CD5- B cells is a characteristic feature in MS.
- CD5+ B cells may be associated with reduced anti-myelin antibody production in the MS central nervous system.