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Related Experiment Videos

Spo13 regulates cohesin cleavage.

Brian H Lee1, Angelika Amon, Susanne Prinz

  • 1Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Genes & Development
|July 9, 2002
PubMed
Summary

Budding yeast Spo13 protein prevents premature loss of cohesin during meiosis I. Overexpressing SPO13 in mitosis inhibits cell division by delaying anaphase onset through blocking cohesin cleavage.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Meiotic chromosome segregation relies on precise cohesin dissociation.
  • Spo13 is crucial for maintaining centromeric cohesin during meiosis I in budding yeast.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying metaphase arrest caused by SPO13 overexpression during mitosis.
  • To understand how Spo13 influences cohesin regulation and anaphase onset.

Main Methods:

  • Investigated the effects of SPO13 overexpression on cell cycle progression in budding yeast.
  • Analyzed the degradation of anaphase inhibitors and cleavage of cohesin subunits.

Main Results:

  • SPO13 overexpression inhibits anaphase onset via two pathways: delaying Pds1 degradation and blocking Esp1-mediated cleavage of Scc1/Mcd1 and Rec8.
  • Spo13 specifically protects cohesin subunits from separase activity, as demonstrated by the unhindered cleavage of another Esp1 substrate, Slk19.

Conclusions:

  • SPO13 overexpression imposes metaphase arrest by inhibiting key events preceding anaphase.
  • Spo13 functions by selectively shielding cohesin complexes from separase-mediated cleavage, thereby ensuring proper chromosome segregation.

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