Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in cAMP levels modulates adhesion to fibronectin and immunostimulatory ability of human dendritic cells.

Dalia Burzyn1, Carolina C Jancic, Sandra Zittermann

  • 1Laboratorio de Inmunogenética, Hospital de Clínicas "José de San Martín", Facultad de Medicina, Universidad de Buenos Aires, Avenida Córdoba 2351 (C.P. 1120), Buenos Aires, Argentina.

Journal of Leukocyte Biology
|July 9, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small Messengers: Glioblastoma-Derived Extracellular Vesicles Modulate γδ T Lymphocytes Through a MIC-Dependent Mechanism.

Biology·2026
Same author

MDX-2001-101 study protocol: a phase I/IIa, multicenter, first-in-human, open-label clinical trial evaluating MDX2001 monotherapy in patients with advanced solid tumors.

Future oncology (London, England)·2026
Same author

Use of research-grade radioisotopes for in-human recovery and survival studies.

Transfusion·2025
Same author

γδ T cells in Escherichia coli infection.

International review of cell and molecular biology·2025
Same author

Microglial depletion prevents visual deficits and retinal ganglion cell loss induced by early life stress in adult animals.

Scientific reports·2025
Same author

Characterization of azithromycin-resistant <i>Shigella flexneri</i> serotype 2a isolates using whole genome sequencing in Ontario from 2016 to 2018.

Microbiology spectrum·2024
Same journal

Distinct lymphocyte immune signatures to nivolumab and recombinant IL-7 ex vivo in patients with sepsis.

Journal of leukocyte biology·2026
Same journal

Eosinophil Essentials: Identification and Assessment of Activity.

Journal of leukocyte biology·2026
Same journal

Immune cell landscape reveals 5 immune-related subtypes and molecular characteristics with prognostic and therapeutic implications in pan-cancer.

Journal of leukocyte biology·2026
Same journal

High-dimensional single-cell mass cytometry analysis of IL-4 treated human macrophages reveals heterogeneity and a CD206high anticolitic subset.

Journal of leukocyte biology·2026
Same journal

SHIP Blocks (MDP+LPS)-Induced Synergy in Macrophages Independent of Catalytic Activity.

Journal of leukocyte biology·2026
Same journal

CD43: Recent Advances and Emerging Functions.

Journal of leukocyte biology·2026
See all related articles

Tumor necrosis factor alpha (TNF-alpha) reduces monocyte-derived dendritic cell (moDC) adhesion to fibronectin and decreases cAMP levels. Cyclic AMP (cAMP) signaling is crucial for TNF-alpha

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Tumor necrosis factor alpha (TNF-alpha) is a key cytokine in immune responses.
  • Monocyte-derived dendritic cells (moDC) play a critical role in initiating adaptive immunity.
  • Cell adhesion and intracellular signaling pathways are vital for moDC function.

Purpose of the Study:

  • To investigate the effects of TNF-alpha on moDC adhesion to fibronectin (FN).
  • To elucidate the involvement of cyclic adenosine monophosphate (cAMP) in TNF-alpha-mediated signaling during moDC adhesion.
  • To analyze the correlation between cAMP levels, moDC adhesion, and allostimulatory capacity.

Main Methods:

  • Treatment of moDC with TNF-alpha and measurement of intracellular cAMP levels.
  • Assessment of moDC adhesion to fibronectin.

Related Experiment Videos

  • Use of phosphodiesterase (PDE) inhibitors and cAMP analogs to modulate signaling.
  • Analysis of IL-12 content and allostimulatory capacity of adhered and non-adhered moDC.

    • Main Results:

    • TNF-alpha treatment decreased both moDC adhesion to FN and intracellular cAMP concentrations.
    • An inverse dose-dependent relationship was observed between TNF-alpha concentration and both adhesion and cAMP levels.
    • Inhibition of PDE or addition of cAMP analogs reversed TNF-alpha-induced effects on adhesion and cAMP.
    • Non-adhered moDC exhibited lower cAMP levels but higher IL-12 content and allostimulatory capacity compared to adhered moDC.
    • The enhanced allostimulatory capacity of non-adhered moDC was diminished by cAMP analogs and PDE inhibitors.

    Conclusions:

    • cAMP signaling plays a significant role in TNF-alpha-induced modulation of moDC adhesion to fibronectin.
    • cAMP levels are inversely correlated with TNF-alpha-induced changes in moDC adhesion and allostimulatory function.
    • These findings highlight the intricate relationship between TNF-alpha, cAMP, and moDC behavior in immune regulation.