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Related Experiment Videos

Epimerization study on [18f]FDG produced by an alkaline hydrolysis on solid support under stringent conditions.

C Mosdzianowsk1, C Lemaire, F Simoens

  • 1Coincidence Technologies s.a., Parc Scientifique du Sart Tilman, Liège, Belgium. mos@coincidence.com

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
|July 10, 2002
PubMed
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Routine synthesis of Fluorine-18 Fluorodeoxyglucose ([18F]FDG) using alkaline hydrolysis on solid support is effective. This method prevents epimerization to [18F]FDM, ensuring high purity of the final [18F]FDG product.

Area of Science:

  • Radiochemistry
  • Nuclear Medicine
  • Organic Synthesis

Background:

  • Solid-phase extraction (SPE) cartridges are widely used for purifying radiolabeled compounds.
  • Alkaline hydrolysis is a common step in the synthesis of [18F]FDG, a crucial positron emission tomography (PET) imaging agent.
  • Concerns exist regarding potential epimerization during alkaline hydrolysis, which could affect product purity.

Purpose of the Study:

  • To investigate the potential for epimerization of [18F]FDG to [18F]FDM during alkaline hydrolysis on a solid support.
  • To determine the impact of high NaOH concentration and extended hydrolysis time on [18F]FDG purity.

Main Methods:

  • Routine synthesis of [18F]FDG using alkaline hydrolysis on a tC18 SPE cartridge.
  • Purification and hydrolysis of the labeled intermediate within the cartridge at room temperature.

Related Experiment Videos

  • Analysis of the final product for the presence of [18F]FDM under various conditions (12 N NaOH, 1 h hydrolysis).

    • Main Results:

    • No epimerization of [18F]FDG to [18F]FDM was observed, even with 12 N NaOH and 1-hour hydrolysis.
    • The alkaline hydrolysis on solid support method consistently yields [18F]FDG with high purity.
    • The method is robust and reliable for routine [18F]FDG production.

    Conclusions:

    • Alkaline hydrolysis on a solid support is a safe and effective method for synthesizing high-purity [18F]FDG.
    • The risk of epimerization to [18F]FDM is negligible under the described conditions.
    • This validated method simplifies [18F]FDG production for clinical and research applications.