Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CUSP/p63 expression in basal cell carcinoma.

Robert P Dellavalle1, Patrick Walsh, Angela Marchbank

  • 1Department of Dermatology, University of Colorado School of Medicine, Denver, Colorado, USA.

Experimental Dermatology
|July 10, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combined pharmacotherapy and psychotherapy impact on opioid use disorder treatment.

The American journal of managed care·2026
Same author

Chromoblastomycosis.

The New England journal of medicine·2026
Same author

When effective anticancer therapies are, in fact, destabilizing the tumor's Group Phenotypic Composition.

NPJ precision oncology·2026
Same author

Multicenter Clinical Comparison of the 10-Minute AMDI Fast PCR Mini Respiratory Panel and the Cepheid Xpert Xpress CoV-2/Flu/RSV plus.

The Journal of molecular diagnostics : JMD·2026
Same author

Predation risk shapes among- and within-individual variation in behavior in poison frog tadpoles.

Behavioral ecology : official journal of the International Society for Behavioral Ecology·2026
Same author

Properdin deficiency or anti-properdin treatment ameliorates disease in the C3 gain-of-function mouse model of atypical haemolytic uraemic syndrome.

Frontiers in immunology·2026
Same journal

52nd Annual Meeting of the Arbeitsgemeinschaft Dermatologische Forschung (ADF).

Experimental dermatology·2026
Same journal

Adverse Event Profile of Apremilast: Pharmacovigilance Study Based on FDA Adverse Event Reporting System (FAERS).

Experimental dermatology·2026
Same journal

Clinical and Immunological Characteristics of Bullous Pemphigoid Patients With Psoriasis Comorbidity: A Retrospective Study.

Experimental dermatology·2026
Same journal

Icariin Regulates Acne Pathophysiology and Extracellular Matrix Protein Synthesis: Implications for Acne Vulgaris and Atrophic Acne Scar Management.

Experimental dermatology·2026
Same journal

Photo-Pollution Induced Activation of the Aryl Hydrocarbon Receptor Drives Oxidative Stress and Melanogenesis in Human Skin Models.

Experimental dermatology·2026
Same journal

Integrated Analysis of Macrophage-Associated Necroptosis in Alopecia Areata.

Experimental dermatology·2026
See all related articles

Chronic ulcerative stomatitis protein (CUSP) is often undetectable in skin cancers like basal cell carcinoma (BCC) and conditions linked to the sonic hedgehog (SHH) pathway. This lack of CUSP/p63 immunofluorescence occurs despite the protein being present and unmutated in these lesions.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Oncology

Background:

  • Chronic ulcerative stomatitis protein (CUSP) is a p63 isoform crucial for epithelial development.
  • CUSP lacks a transactivation domain and can inhibit p53 function.
  • Its role in skin neoplasms, particularly those involving the sonic hedgehog (SHH) pathway, is not well understood.

Purpose of the Study:

  • To investigate the expression and immunofluorescence of CUSP/p63 in various cutaneous neoplasms.
  • To determine if CUSP/p63 expression is altered in conditions associated with SHH pathway dysregulation, such as basal cell carcinoma (BCC).

Main Methods:

  • Immunohistochemical analysis of CUSP/p63 expression in biopsies of normal skin, benign neoplasms, squamous cell carcinoma (SCC), and basal cell carcinoma (BCC).

Related Experiment Videos

  • Analysis of skin samples from patients with basal cell nevus syndrome (BCNS) and a mouse model of BCC.
  • Detection of CUSP mRNA and protein via Northern and Western blot analysis.
  • Sequencing of the CUSP coding region in BCC samples lacking immunofluorescence.

    • Main Results:

    • Strong nuclear CUSP immuno-reactivity was observed in normal epidermis, SCC, seborrheic keratosis, acrochordon, and verruca plana.
    • Few BCCs and sebaceous nevi displayed CUSP/p63 immuno-reactivity.
    • Cutaneous conditions with SHH pathway dysregulation (BCC, BCNS, sebaceous nevi) were significantly more likely to lack CUSP/p63 immunofluorescence (over 86 times).
    • CUSP mRNA and protein were detected in BCCs lacking immunofluorescence, and no mutations were found in the CUSP coding region.

    Conclusions:

    • CUSP/p63 is frequently undetectable by immunofluorescence in cutaneous lesions associated with SHH pathway dysregulation, including BCC and BCNS.
    • This lack of detection occurs despite the presence and lack of mutation in the CUSP protein.
    • CUSP/p63 immunofluorescence may serve as a biomarker for SHH pathway activity in skin neoplasms.