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Optimisation of the degenerate oligonucleotide primed PCR (DOP-PCR) for capillary thermocycler
Csaba Kiss1, Maria Kost-Alimova, George Klein
1Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Nobels vag 16, S-17177 Stockholm, Sweden. csaba.kiss@mtc.ki.se
Biomolecular Engineering
|July 10, 2002
Abstract:
DOP-PCR was optimised for capillary thermocycler. Five primers with five or six nucleotide anchor sequences and different restriction enzyme recognition sites were tested. The efficiency of the amplification was confirmed by gel electrophoresis and fluorescence in situ hybridisation on metaphase chromosomes.