Single Channel Recording from Glial Cells on the Untreated Surface of the Frog Optic Nerve

The patch clamp technique has been used to record single channel currents from the untreated surface of the intact frog optic nerve after the meninges and basal lamina have been mechanically removed. Cells filled via dialysis with Lucifer yellow (LY) from the patch pipette had a typical astrocyte morphology and were dye-coupled to adjacent astrocytes. This is consistent with the electron-microscopic observation that all the cells on the surface of this nerve are astrocytes. Two types of ion channels were studied in detached patches. One, identified as a K+ channel, had a conductance of 88 +/- 4 (S.E.) n=9 pS and an equilibrium potential of -59 +/- 8 mV in physiological K+ solutions. The steady-state open probability was not significantly altered by changing the membrane potential. A second channel had a large conductance of 300 - 1200 pS, a reversal potential of approximately 0 mV in symmetrical and non-symmetrical solutions, and was open only in the voltage range of +/-20 mV. These are the characteristics of a large anionic channel described in other preparations including cultured astrocytes.