Modeling the pathways of energy balance using the N1E-115 murine neuroblastoma cell line
Jonathan D Roth1, Daniel K Yee, Lori R Kisley
1Department of Animal Biology, School of Vetrinary Medicine and the Institute of Neurological Sciences, University of Pennsylvania, Philadelphia, PA 19104, USA. jdroth@vet.upenn.edu
Abstract:
A good in vitro model within which to investigate molecular interactions between feeding relevant neuropeptide systems has been lacking. Consequently, we began using reverse transcriptase-polymerase chain reaction (RT-PCR) to screen various neuronal cell lines for the presence of feeding relevant neuropeptides and receptors. N1E-115 murine neuroblastoma cells have emerged as an attractive candidate for further analysis because they contain mRNA for a variety of key systems implicated in the regulation of energy homeostasis.
