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No decrease in myonuclear number after long-term denervation in mature mice.

K-I Wada1, H Takahashi, S Katsuta

  • 1Laboratory of Exercise Biochemistry, Institute of Health and Sport Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8574, Japan.

American Journal of Physiology. Cell Physiology
|July 11, 2002
PubMed
Summary
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Muscle fiber atrophy in mature mice due to denervation does not decrease myonuclear number. Age impacts how myonuclear number changes during muscle fiber atrophy.

Area of Science:

  • Muscle physiology
  • Cell biology
  • Aging research

Background:

  • Muscle fiber atrophy can occur with aging or denervation.
  • Previous studies on myonuclear number changes during atrophy are controversial.
  • The role of age in denervation-induced myonuclear dynamics is unclear.

Purpose of the Study:

  • To investigate age-related differences in myonuclear number changes during denervation-induced muscle fiber atrophy.
  • To compare the effects of denervation on young versus mature mouse skeletal muscle.

Main Methods:

  • Denervation of plantaris muscles in young (3-wk-old) and mature (4-mo-old) mice.
  • Isolation of single myofibers using alkali maceration.
  • Analysis of fiber cross-sectional area (CSA), myonuclear number, and cytoplasm-to-myonucleus (C/N) ratio.

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Main Results:

  • Denervation reduced fiber CSA in both young and mature mice.
  • Myonuclear number decreased in young mice post-denervation but remained unchanged in mature mice.
  • Cytoplasm-to-myonucleus ratio decreased in mature mice, but not in young mice.

Conclusions:

  • Age significantly influences the change in myonuclear number during denervation-induced muscle atrophy.
  • Skeletal muscle cytoplasmic atrophy can occur independently of myonuclear number reduction, particularly in mature animals.