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Pendrin is an iodide-specific apical porter responsible for iodide efflux from thyroid cells
Akio Yoshida1, Shinichi Taniguchi, Ichiro Hisatome
1First Department of Internal Medicine, Tottori University Faculty of Medicine, Nishimachi 36-1, Yonago, Tottori 683-8504, Japan. ayoshida@bronze.ocn.ne.jp
The Journal of Clinical Endocrinology and Metabolism
|July 11, 2002
Summary
Pendrin, a protein encoded by the Pendred syndrome gene, functions as an iodide-specific transporter in mammalian cells. This finding clarifies its crucial role in thyroid iodide efflux.
Area of Science:
- Endocrinology
- Molecular Biology
- Genetics
Background:
- Pendred syndrome is linked to mutations in the Pendred syndrome gene, which encodes pendrin.
- Pendrin is a transmembrane protein found in thyroid follicular cells.
- Previous studies demonstrated pendrin's transport of iodide and chloride in non-mammalian systems.
Purpose of the Study:
- To investigate the function of pendrin as an iodide transporter in mammalian cells.
- To elucidate the role of pendrin in thyroid iodide transport.
Main Methods:
- Transfection of COS-7 and Chinese hamster ovary cells with human Pendred syndrome gene cDNA.
- Expression of sodium iodide symporter alongside pendrin.
- Comparative analysis with studies using rat thyroid FRTL-5 cells.
Main Results:
- Pendrin demonstrated iodide-specific transport activity in mammalian cells.
- Pendrin was confirmed to be responsible for iodide efflux in the thyroid.
- Mammalian cell studies provide a relevant model for thyroid pendrin function.
Conclusions:
- Pendrin is an iodide-specific transporter in mammalian cells.
- Pendrin plays a key role in thyroid iodide efflux.
- This study validates pendrin's function in a mammalian context relevant to thyroid physiology.