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Updated: Nov 26, 2025

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
Published on: November 4, 2019
Fusion Expression and Antibody Preparation of the Human Transcription Factor hB1F
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. wangyuan@server.shcnc.ac.cn
Abstract:
Human B1F was cloned from a human liver cDNA library by yeast one-hybrid screening and characterized. It is a transcription factor which belongs to nuclear receptor superfamily. The cDNA segment 450-930 of human liver transcription factor hB1F was cloned into the pGEX-3X expression vector and was expressed in E.coli. The purified GST-fused hB1F, named GST-CS, were used to immunize mice to get polyclonal antibodies to hB1F. The antiserum showed specificity to hB1F after the purification by GST-Sepharose 4B. It can be used in further investigations of the structure and function of hB1F.
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