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Published on: February 1, 2011
Adsorption on Cellulose of the Schizophyllum commune Cellobiose Dehydrogenase
1The State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China. gaopj@life.sdu.edu.cn
Abstract:
Equilibrium of adsorption of cellobiose dehydrogenase (CDH) of Schizophyllum commune on cotton cellulose, microcrystalline cellulose, and phosphate-swollen cellulose was reached within 4 min. The binding isotherms of CDH adsorbed on the above substrates were a straight line in Scatchard plot analysis, indicating that the binding fitted to the one-binding-site model. It was found that CDH adsorption onto microcrystalline cellulose was strongly affected by temperature, pH, NaCl, and ethylene glycol, respectively.
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