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Updated: Sep 30, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Diformylhydrazine as analytical reagent for spectrophotometric determination of iron(II) and iron(III)
B M Nagabhushana1, G T Chandrappa, B Nagappa
1Department of Chemistry, M.S. Ramaiah Institute of Technology, Bangalore-560054, India.
Abstract:
The bidentate ligand diformylhydrazine (OHC-HN-NH-CHO), DFH, combines with iron(II) and iron(III) in alkaline media in the pH range 7.3-9.3 to form an intensely colored red-purple iron(III) complex with an absorption maximum at 470 nm. Beer's law is obeyed for iron concentrations from 0.25 to 13 microg mL(-1). The molar absorptivity was in the range 0.3258x10(4)-0.3351x10(4) L mol(-1) cm(-1) and Sandell's sensitivity was found to be 0.0168 microg cm(-2). The method has been applied to the determination of iron in industrial waste, ground water, and pharmaceutical samples.
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