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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
Published on: September 22, 2011
Industrial processes with animal cells
1Institut für Technische Chemie der Universität Hannover, Callinstrasse 3, Germany. Kretzmer@iftc.uni-hannover.de
Applied Microbiology and Biotechnology
|July 12, 2002
Summary
Industrial animal cell culture has advanced from vaccines to complex glycoproteins. Process development is product-dependent, ranging from small-scale reactors to large bioreactors, with perfusion culture facing challenges.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Cell Culture Technology
Background:
- Industrial applications of animal cell culture have grown significantly over the past four decades.
- Initial processes focused on virus vaccines, evolving to include monoclonal antibodies and complex glycoproteins.
- Advancements in permanent cell lines and chemically defined media enabled large-scale cultivation.
Purpose of the Study:
- To review the historical development and current state of industrial animal cell culture processes.
- To discuss the impact of key technological advancements like hybridomas and genetic engineering.
- To analyze the challenges and scalability considerations for different production systems.
Main Methods:
- Historical review of biopharmaceutical production technologies.
- Analysis of cell line development and culture medium evolution.
- Comparison of small-scale (multiple-unit) and large-scale (single-unit) bioreactor systems.
Main Results:
- Significant increase in the diversity and complexity of products derived from animal cell cultures.
- Development of hybridoma technology and genetic engineering enabling new therapeutic and diagnostic products.
- Production scales vary widely, from small reactors for high-value, low-demand products to large bioreactors for bulk products.
Conclusions:
- Animal cell culture is a critical technology for producing a range of biopharmaceuticals.
- Process development is dictated by product characteristics, influencing scale and system choice.
- Further development is needed to overcome challenges in implementing effective perfusion cultures for industrial bioprocessing.
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