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Industrial processes with animal cells.

G Kretzmer1

  • 1Institut für Technische Chemie der Universität Hannover, Callinstrasse 3, Germany. Kretzmer@iftc.uni-hannover.de

Applied Microbiology and Biotechnology
|July 12, 2002
PubMed
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Industrial animal cell culture has advanced from vaccines to complex glycoproteins. Process development is product-dependent, ranging from small-scale reactors to large bioreactors, with perfusion culture facing challenges.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Culture Technology

Background:

  • Industrial applications of animal cell culture have grown significantly over the past four decades.
  • Initial processes focused on virus vaccines, evolving to include monoclonal antibodies and complex glycoproteins.
  • Advancements in permanent cell lines and chemically defined media enabled large-scale cultivation.

Purpose of the Study:

  • To review the historical development and current state of industrial animal cell culture processes.
  • To discuss the impact of key technological advancements like hybridomas and genetic engineering.
  • To analyze the challenges and scalability considerations for different production systems.

Main Methods:

  • Historical review of biopharmaceutical production technologies.

Related Experiment Videos

  • Analysis of cell line development and culture medium evolution.
  • Comparison of small-scale (multiple-unit) and large-scale (single-unit) bioreactor systems.

    • Main Results:

    • Significant increase in the diversity and complexity of products derived from animal cell cultures.
    • Development of hybridoma technology and genetic engineering enabling new therapeutic and diagnostic products.
    • Production scales vary widely, from small reactors for high-value, low-demand products to large bioreactors for bulk products.

    Conclusions:

    • Animal cell culture is a critical technology for producing a range of biopharmaceuticals.
    • Process development is dictated by product characteristics, influencing scale and system choice.
    • Further development is needed to overcome challenges in implementing effective perfusion cultures for industrial bioprocessing.