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Coupled lactic acid fermentation and adsorption.

C Chen1, L-K Ju

  • 1Department of Chemical Engineering, University of Akron, OH 44325-3906, USA.

Applied Microbiology and Biotechnology
|July 12, 2002
PubMed
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Activated carbon effectively removes lactic acid during fermentation, doubling production. This method also immobilizes Lactobacillus delbrueckii subsp. delbrueckii cells, but requires yeast extract for sustained activity.

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Fermentation Technology

Background:

  • Lactic acid fermentation is crucial for various industries.
  • High product concentrations can inhibit microbial growth and reduce yields.
  • Adsorption methods can mitigate product inhibition by removing lactic acid from the broth.

Purpose of the Study:

  • To evaluate polyvinylpyridine (PVP) and activated carbon for coupled lactic acid fermentation and adsorption.
  • To investigate activated carbon for simultaneous cell immobilization and lactate adsorption.
  • To determine the optimal conditions for sustained lactic acid production using activated carbon.

Main Methods:

  • Lactic acid fermentation coupled with periodical circulation through PVP and activated carbon adsorption columns.

Related Experiment Videos

  • Adsorbent regeneration using 0.1 N NaOH.
  • Semi-batch fermentation with periodical medium replacement using activated carbon for cell immobilization and lactate adsorption.
  • Investigating the effect of yeast extract concentration on lactic acid production.

    • Main Results:

    • PVP adsorption doubled lactic acid production but suffered capacity loss during regeneration.
    • Activated carbon showed superior adsorption of lactic acid and lactate compared to PVP.
    • Activated carbon effectively immobilized Lactobacillus delbrueckii subsp. delbrueckii cells.
    • Sustained lactate production of 1.3 g/l/h was achieved with activated carbon when yeast extract was present at ≥2 g/l.

    Conclusions:

    • Activated carbon is a more effective and practical adsorbent for lactic acid fermentation than PVP.
    • Simultaneous cell immobilization and lactate adsorption using activated carbon is a viable strategy.
    • Yeast extract is essential for sustained lactic acid production by Lactobacillus delbrueckii subsp. delbrueckii, with a critical level below 2 g/l.