Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Method for quantitating sensitivity to a staphylococcal bacteriocin.

G Van Norman, N Groman

    Infection and Immunity
    |November 1, 1979
    PubMed
    Summary

    A new method efficiently measures bacterial sensitivity to bacteriocins. This technique is valuable for analyzing large bacterial collections, including Corynebacterium diphtheriae, against Staphylococcus aureus bacteriocins.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Optimal propofol-alfentanil combinations for supplementing nitrous oxide for outpatient surgery.

    Anesthesiology·1999
    Same author

    Propofol versus isoflurane for endoscopic sinus surgery.

    American journal of otolaryngology·1999
    Same author

    DNA sequence homology between attB-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and the attP site of gamma-corynephage.

    FEMS microbiology letters·1990
    Same author

    Transformation of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and Escherichia coli with the C. diphtheriae plasmid pNG2.

    Proceedings of the National Academy of Sciences of the United States of America·1987
    Same author

    Detection of homology to the beta bacteriophage integration site in a wide variety of Corynebacterium spp.

    Journal of bacteriology·1986
    Same author

    DNA relationships among some tox-bearing corynebacteriophages.

    Infection and immunity·1985

    Area of Science:

    • Microbiology
    • Bacteriology
    • Antimicrobial Resistance

    Background:

    • Bacteriocins are antimicrobial peptides produced by bacteria.
    • Assessing bacterial susceptibility to bacteriocins is crucial for understanding microbial interactions and developing novel antimicrobials.
    • Corynebacterium diphtheriae poses public health challenges, necessitating methods to study its susceptibility to antibacterial agents.

    Purpose of the Study:

    • To describe a convenient and efficient method for quantifying the sensitivity of bacterial strains to bacteriocins.
    • To demonstrate the utility of this method for large-scale screening of bacterial populations.
    • To specifically assess the sensitivity of Corynebacterium diphtheriae to a Staphylococcus aureus bacteriocin.

    Main Methods:

    • Development of a quantitative assay for bacteriocin sensitivity testing.
    • Application of the method to a large number of bacterial strains.
    • Utilizing Staphylococcus aureus phage type 71 bacteriocin as the test agent.

    Main Results:

    • A convenient method for quantitating bacterial sensitivity to bacteriocins was successfully established.
    • The method allows for the analysis of large bacterial strain collections.
    • Initial application focused on Corynebacterium diphtheriae sensitivity to a specific Staphylococcus aureus bacteriocin.

    Conclusions:

    • The described method provides a practical approach for determining bacteriocin sensitivity in bacteria.
    • This technique facilitates high-throughput screening for antimicrobial susceptibility.
    • Further studies can utilize this method to investigate bacteriocin activity against various bacterial pathogens, including Corynebacterium diphtheriae.

    Related Experiment Videos