Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microarray-assisted screen for potential Hap1 and Rox1 target genes in Saccharomyces cerevisiae.

José J M Ter Linde1, H Yde Steensma

  • 1Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. steensma@rulbim.leidenuniv.nl

Yeast (Chichester, England)
|July 12, 2002
PubMed
Summary

Saccharomyces cerevisiae gene expression adapts to oxygen levels via Hap1 and Rox1 transcription factors. This study identifies new target genes for Hap1 and Rox1, revealing their roles in oxygen-regulated gene control.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiplex spatial omics reveals changes in immune-epithelial crosstalk during inflammation and dysplasia development in chronic IBD patients.

iScience·2024
Same author

Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis.

iScience·2023
Same author

Compartment-driven imprinting of intestinal CD4 T cells in inflammatory bowel disease and homeostasis.

Clinical and experimental immunology·2023
Same author

Conjunctival inflammation in dupilumab-treated atopic dermatitis comprises a multicellular infiltrate with elevated T1/T17 cytokines: A case series study.

Allergy·2021
Same author

Functional dyspepsia patients have lower mucosal cholecystokinin concentrations in response to duodenal lipid.

European journal of gastroenterology & hepatology·2013
Same author

In gastroesophageal reflux disease, differential gene expression in the duodenum points towards enhanced chylomicron production and secretion.

Digestive diseases and sciences·2012

Area of Science:

  • * Molecular biology
  • * Yeast genetics
  • * Gene regulation

Background:

  • * Saccharomyces cerevisiae exhibits differential gene expression in response to oxygen availability.
  • * Oxygen control of gene expression under aerobic conditions involves the activator Hap1 and repressor Rox1.
  • * Hap1 senses heme status and upregulates aerobic genes, while also activating ROX1 transcription to repress hypoxic genes.

Purpose of the Study:

  • * To identify novel Hap1 and Rox1 target genes using genome-wide expression analysis.
  • * To further elucidate the regulatory roles of Hap1 and Rox1 in oxygen adaptation.
  • * To investigate Rox1's function in repressing anaerobic genes under normoxic conditions.

Main Methods:

  • * Microarray analysis of gene expression in aerobically cultivated hap1 and rox1 null mutants.

Related Experiment Videos

  • * Transcriptome data from aerobic and anaerobic growth conditions.
  • * Computational promoter analysis to identify transcription factor binding sites.

    • Main Results:

    • * Deletion of HAP1 resulted in decreased transcript levels for 51 genes.
    • * Transcription of 40 genes was increased in rox1 mutant cells compared to wild-type.
    • * Identified 24 potential Hap1 target genes and 38 direct Rox1 target genes.
    • * Provided evidence for Rox1 repressing anaerobic genes under normoxic conditions.

    Conclusions:

    • * Hap1 and Rox1 are key regulators of oxygen-dependent gene expression in Saccharomyces cerevisiae.
    • * This study expands the known targets of Hap1 and Rox1, providing a more comprehensive view of their regulatory networks.
    • * Rox1 plays a significant role in repressing anaerobic gene expression during aerobic growth.