Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amphibia Kupffer cells.

Giovanni Sichel1, Marina Scalia, Concetta Corsaro

  • 1Dipartimento di Scienze Biomediche, Sezione di Biologia generale, cellulare e di Genetica molecolare, Università di Catania, Catania, Italy. sichel@mbox.unict.it

Microscopy Research and Technique
|July 12, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrastructural Sperm Flagellum Defects in a Patient With <i>CCDC39</i> Compound Heterozygous Mutations and Primary Ciliary Dyskinesia/<i>Situs Viscerum Inversus</i>.

Frontiers in genetics·2020
Same author

Ovarian aging increases small extracellular vesicle CD81<sup>+</sup> release in human follicular fluid and influences miRNA profiles.

Aging·2020
Same author

Isolation and Characterization of a New Human Corneal Epithelial Cell Line: HCE-F.

Cornea·2020
Same author

PARP-14 Promotes Survival of Mammalian α but Not β Pancreatic Cells Following Cytokine Treatment.

Frontiers in endocrinology·2019
Same author

LncRNA UCA1, Upregulated in CRC Biopsies and Downregulated in Serum Exosomes, Controls mRNA Expression by RNA-RNA Interactions.

Molecular therapy. Nucleic acids·2018
Same author

Monocytic myeloid-derived suppressor cells as prognostic factor in chronic myeloid leukaemia patients treated with dasatinib.

Journal of cellular and molecular medicine·2017

Amphibia Kupffer cells share many macrophage traits with mammals but uniquely produce melanin. These liver cells in amphibians exhibit distinct features, including melanin synthesis, unlike their mammalian counterparts.

Area of Science:

  • Comparative immunology
  • Cell biology
  • Amphibian physiology

Background:

  • Kupffer cells are liver-resident macrophages crucial for immune responses.
  • Understanding Kupffer cell heterogeneity across species aids in comprehending immune system evolution.
  • Amphibian immune systems offer unique insights due to their evolutionary position.

Purpose of the Study:

  • To compare the ultrastructural and functional characteristics of Kupffer cells in Amphibia and Mammalia.
  • To identify conserved and divergent features of these macrophages.
  • To investigate the unique capacity of amphibian Kupffer cells for melanin production.

Main Methods:

  • Comparative ultrastructural analysis of Kupffer cells.
  • Histochemical staining for enzyme activity (e.g., nonspecific esterase, peroxidase).

Related Experiment Videos

  • Gene expression analysis for tyrosinase and observation of melanosome formation.

    • Main Results:

    • Amphibian and mammalian Kupffer cells share common features like filopodia, phagocytic activity, and esterase presence.
    • Distinct differences include the presence of phagocytosed red cells, iron-protein complexes, and lipofuscin in amphibian Kupffer cells.
    • Amphibian Kupffer cells uniquely express tyrosinase, possess "melanosome centers," and show dopa oxidase activity, indicating melanin production.

    Conclusions:

    • Amphibian Kupffer cells exhibit a unique specialization in melanin synthesis, a trait absent in mammals.
    • Despite conserved macrophage functions, significant species-specific adaptations exist in liver-resident macrophages.
    • These findings highlight the diverse roles and evolutionary pathways of Kupffer cells across vertebrate classes.